Abstract: In order to investigate the shear behavior of high-strength concrete beams with HRB600 steel bars, the monotonic loading tests of five concrete beams with longitudinal high-strength reinforcement were carried out. The main design parameters of test specimens were longitudinal reinforcement ratio and steel fiber contents. The shear load-carrying capacity, load-deflection curve, crack width, failure characteristics of each specimen were compared. The test results show that the cracking load and ultimate shear load of high-strength concrete beams increase with the increase of longitudinal reinforcement ratio, but the width of diagonal cracks decreases; the steel fiber can effectively improve the cracking load of high-strength concrete beams and limit the generation and development of diagonal cracks; with the increase of steel fiber content, the shear load-carrying capacity of high-strength concrete beams increases; the shear load-carrying capacity of test beams was calculated using the current code for design of concrete structures and technical specification for application of fiber reinforced concrete, and the calculated results were less than the measured results.