Abstract: Free standing stele relics is a common form of stele display in museum for aesthetics and easy maintenance. However, the relics are prone to damage during earthquakes because of inadequate seismic fortification of the museums. Seismic isolation is an effective measure to improve the seismic performance of the museum facilities and protect the stele relics. To guide the seismic design of the museum facilities, studied were the seismic responses of the stele relics and the seismic isolation fortification of host buildings. The current specification considers too little the impact of vertical earthquake and the deformation of the stele bottom. Thusly, proposed is a more reasonable limit of the horizontal acceleration of free-standing stele relics. Through theoretical analysis, studied are the allowable acceleration spectra of stele relics under harmonic excitation, and selected is the minimum value of the allowable acceleration spectrum in the designated frequency range for the floor vibration of base isolation buildings as the design limit of the floor acceleration. It is determined that the allowable acceleration spectrum derived from theoretical analysis is more accurate and conservative than current specification through finite element analysis. Designing the isolation system of buildings upon the seismic fortification limit proposed is effective in protecting the free-standing stele relics from overturning.