Abstract: A study was conducted on the high-frequency fatigue life evaluation of engine combustion chamber structures in thermal-acoustic environment. Based on the hybrid finite element-statistical energy analysis method, the zero-order moment stress spectrum method and the Dirlik method considering the mean stress effect, a high-frequency fatigue life evaluation method that comprehensively considers multiple thermal effects is proposed. The influence of material elastic modulus, S-N curve, thermal stress and acoustic load on the high-frequency fatigue life of the chamber structure is evaluated. The results indicate that for the engine combustion chamber structure studied in this paper, with the increase of temperature, the reduction of material elastic modulus leads to the increase of structural fatigue life. The material fatigue performance becomes worse, which result in the reduction of the structural fatigue life. While the increasing structural thermal stress leads to a decrease in the fatigue life. The change in material S-N curve has the greatest influence on the fatigue life of the structure, followed by the change of elastic modulus and the change of thermal stress. Meanwhile, the higher the level of acoustic loads, the lower the fatigue life of the structure.