Abstract: To study the punching shear resistance of slab-column connections with partial engineered cementitious composites (ECC) replacement, two connections with different replacement areas and one RC connection were tested under quasi-static loading. The test results indicate that partially replaced concrete with ECC can significantly increase the ductility of the connection and convert the failure mode from punching shear failure to flexural-punching failure. Moreover, numerical analyses were conducted to investigate the effects of replacement area, slab thickness and compressive strength of ECC on the punching shear resistance of slab-column connections. The results demonstrate that when the ECC replacement length is one times the slab thickness from the column surface, the punching shear resistance and deformation capacity can be increased by 42% and 227%, respectively. However, further increasing the replacement length can only slightly increase the punching shear resistance and deformation capacity of the connections. Thus, the replacement length is suggested to be one times the slab thickness from column surface. Increasing the slab thickness can significantly improve the punching shear resistance and initial stiffness of slab-column connections while the increase of ECC compressive strength has little effect on it.