Abstract: The installation of tuned mass dampers (TMD) is an effective method to reduce human-induced vibration of large-span floors. The parameters and locations of the TMDs are usually empirically determined, which makes it difficult to ensure the most optimized result and to consider the stochastic feature of the human-induced load. A strategy for human-induced vibration serviceability design is proposed based on topology optimization. The stochastic feature of the human-induced vibration is considered, and the optimization constraints are determined from the perspective of dynamic reliability constraints. Satisfactory results are obtained by applying the method to the optimal design of the human-induced vibration using TMDs for a large-span structure.