Abstract: In order to realize the quantitative analysis of pedestrian-induced vibration comfort, the concepts of "human body resistance" and "vibration effect" are established according to the principle of probability statistics, and the mathematical definition of sensitivity is derived. Based on the field experiments on Huatai Footbridge, the relationships between human perception coefficient and natural vibration frequency, peak acceleration, pedestrian comfort coefficient, pedestrian density and comfort membership degree are analyzed. The quantitative calculation formula of comfort is proposed according to sensitivity and membership function. The results show that: there is no significant correlation between the human perception coefficient and the natural vibration frequency, and the peak acceleration has the highest correlation with pedestrian density, showing a positive proportional relationship. The measured comfort is compared with the calculated comfort, the errors are 8.6%, 13.4%, 11.6% and 12.7% under the four working conditions with pedestrian density T_c = 0.2, 0.4, 0.8 and 1.2/m², and the comfort grades are consistent.