Abstract: During interventional procedures, the interaction between surgical needles and soft tissues induces substantial deformation of the soft tissues, leading to displacement of the target lesions and consequently compromising procedural accuracy. Developing a precise soft tissue deformation model is of critical theoretical importance for preoperative prediction or intraoperative manipulation of lesion sites. This paper innovatively proposes the use of a honeycomb structure to characterize the mechanical properties and deformation behavior of soft tissues. A honeycomb unit cell suitable for representing soft tissues is constructed, and its geometric composition and topological configuration are elaborated. Based on Euler-Bernoulli beam theory and by integrating the force method regularization, Mohr’s integral, and the energy approach, the analytical expressions for displacement and the equivalent Young’s modulus of the unit cell under compressive loading are derived. The theoretical model is further validated through finite element simulations. Via material compression experiments on biomimetic soft tissues, an ideal honeycomb cell with matching mechanical properties is identified, thereby establishing a honeycomb-based deformation model capable of effectively describing the mechanical characteristics and deformation behavior of soft tissues.