Abstract: Based on the Euler-Beam theory, the moving vehicle loads on bridges were identified from the response of bridges using the Time Domain Method (TDM) and Frequency-Time Domain Method (FTDM). This paper aims to investigate the effect of various parameters on the two methods. These parameters include mode number, sampling frequency, vehicle speed, locations and numbers of measured stations. Assessment results show that the vehicle-bridge model in laboratory is successful. Both methods are effective and acceptable with higher accuracy, and the TDM is the preferred in-situ method with good feasibility and robustness.